… and coworkers employed Ni 2+ chelating peptides that were … we elucidate the optimal
combination of the peptide sequence, … Amide bond hydrolysis was induced by complexation by the …

… protein chemistry as exemplified by recent reviews [17,20,21,25,[35], [36], [37], [38], [39], [40],
[41]]. The peptide bond hydrolysis initiated by Ni(II… can be optimized for each target protein. …

… Ni(II)-assisted peptide bond hydrolysis of the tested peptides. … peptides in the 4N hydrolytically
active complex (Table 2) and … geometry optimization using a Dreiding-like forcefield [43]. …

… proteins to be hydrolyzed site-specifically by Ni 2+ ions; motif … optimized amino acid
sequence has been developed for Ni (… )-dependent peptide bond hydrolysis (39), a His 6 tag (…

… substrate and protein engineering described in this review. … which catalyse the hydrolysis
of peptide bonds are much … for peptide ligations is that they are inherently sequence specific. …

… Advanced protein engineering of enzymes derived from extremophiles is also … the hydrolysis
of peptide bonds. [ 26 ] This specific Pd(II)–CD complex was shown to be sequence specific, …

… (18−22) This problem can be overcome through the incorporation of the active metal salts …
proven to be active toward hydrolysis of peptide bonds in a range of dipeptides and proteins. (…

… of how Ni(II) substitution alters the DNA sequence-specific … and catalyze the hydrolysis of
peptide bonds between Gln … in various fields, including protein engineering, medicine, and …

… may include the peptide bond hydrolysis in X 1 -S/Tc/pHcX 2 motifs by released Ni 2+ ions.
… 150 mM NaCl buffer with or without nickel ions [1 mM Ni(NO 3 ) 2 ] under optimal (pH 8.2, 50C…

… amide bond of the β-lactam ring, followed by the involvement of an activated water molecule
at Glu166, culminating in the hydrolysis of the ester bond … can further optimize the substrate …